Toosendanin analysis. Feb 15, 2023 · Statistical analysis by one-way ANOVA.

Toosendanin analysis 2016; 6:34225. However, the effects of TSN on glioma cells have rarely been reported. A: Cells transfected with CTPS interference vector for 48 h. b, c The viability of U87MG, LN18, U-251MG, and LN229 glioma cells and normal human astrocytes (SVG p12 cell) treated with different concentrations of TSN (0, 50, 100, 200, and 300 μM) for 24, 48, and 72 h was detected using CCK-8 assay. Methods: Transonic alcohol-chloroform extraction method was used to extract toosendanin from the bark of Melia toosendan Sieb. Cells were washed with pre-cooled PBS to eliminate the unbound virions and incubated at 37°C for 3 h in the presence of ammonium chloride (20 mM), then washed and Toosendanin (TSN) is a major triterpenoid existing in Melia toosendan, which has been used as a digestive tract parasiticide and insecticide but with serious hepatotoxicity. et al. May 6, 2022 · Autophagy has been reported as a pro-survival response to chemotherapeutic drugs in TNBC, and suppression of autophagy can be a strategy to overcome drug resistance. Furthermore, PICRUSt2 analysis found that 16 bacterial function categories were altered by CAR treatment, of which 8 were significantly increased. In preparation for cell cycle analysis, the cells were fixed in 70% pre-cooled ethanol for 24 h and washed with 1 mL PBS for 3 times. Cells were washed with pre-cooled PBS to eliminate the unbound virions and incubated at 37°C for 3 h in the presence of ammonium chloride (20 mM), then washed and Nov 1, 2023 · Toosendanin (TSN), extracted from Melia. Triterpenoid Toosendanin (TSN) exhibits a plenty of pharmacological effects in human and great values in agriculture. Toosendanin in rat plasma was found to be stable after being stored at 25°C for 8 h, −70°C for 30 days, and having gone through three freeze–thaw cycles. However, the mechanism is not clear. In this experiment, a chick model of artificially induced E. , 2016). Our previous research found that covalent modification of TSN for proteins might be a possible reason using human liver microsomes, and t … Nov 20, 2018 · Toosendanin is the main chemical composition of the fructus toosendan. The efficacy of toosendanin (TSN) in blocking autophagy flux was measured by western blot analysis of autophagy markers, and the fluorescent imaging of RFP-GFP-LC3 probe. RNA sequence transcriptome analysis is performed on toosendanin-treated cells to identify target genes and signaling pathways. By applying cellular thermal shift assay (CETSA), immunoprecipitation-coupled LC-MS/MS analysis, and biotin-toosendanin pull-down assay, we confirmed the direct binding between and toosendanin V-ATPase. et Zucc. In recent years, in vitro studies have found that Toosendanin has certain inhibitory effects on breast cancer cells, liver cancer cells, human leukemia cells, and so forth Mar 15, 2018 · Toosendanin (TSN) is the main active sectioned, and subject to hematoxylin-eosin (H & E) staining for histological analysis according to the standard protocols. 1, 2 With an incidence rate of 3. Although the LC–MS method showed obvi-ous advantage for its distinctive sensitivity and selectivity, it can hardly meet the requirement of the routine analysis Jan 1, 2023 · In 1999, two new limonoids, toosendanal (47) and 12-O-methylvolkensin (48) along with three known limonoids, meliatoxin B1 (49), trichilin H (50), and toosendanin (10) were isolated from FMT. Statistical analysis by two-tailed Student Feb 2, 2023 · Toosendanin is a small-molecule compound that blocks macrophage-mediated immunosuppression. To evaluate the effect of TSN on cell cycle and apoptosis, 2. Toosendanin was subjected to similar evaluation against neonate larvae to provide final concentrations of 20 to Means within a column followed by different letters 140 ppm. 34225 Identification of Amino Acid and Glutathione N-Conjugates of Toosendanin: Bioactivation of the Furan Ring Mediated by CYP3A4 J Yu, P Deng, D Zhong, X Chen Chemical Research in Toxicology 27 (9), 1598-1609 , 2014 Cell cycle and apoptosis analysis. 9 Thus, exposure of neurons to BoNT/A in presence of increasing concentrations of toosendanin resulted in the gradual preservation of intact, uncleaved synaptosomal associated Jan 30, 2024 · Toosendanin (TSN) is a botanical compound with broad-spectrum insecticidal activities against many pests. An ultra-performance liquid chromatography-electrospray ionization-mass spectrometry method was developed for determination of … Jun 19, 2018 · Lu X, Ji C, Tong W, Lian X, Wu Y, Fan X, et al. To investigate the immunosuppressive activity of toosendanin on T-cells, human T-cells were labelled with CFSE and flow cytometry was used to detect the effect of toosendanin on the proliferation of T-cells activated by anti-human CD3/CD28 antibodies. Scale bar indicates 100 µm. 8 It has been registered and commercialized in China as an important Chinese traditional insecticide. and Melia. , which is traditionally used for treatment of for the quantitative analysis of toosendanin. Western blot analysis was used to analyze expression levels of apoptosis-related protein Bcl-2, pro-apoptotic Bax, cytochrome c, caspase-3, −8 and −9 and PARP to. Sep 30, 2013 · Further analysis on the MS spectra showed M1, M2, and M3 are oxidative products and M6 is a dehydrogenation product, while M4 and M5 are oxidative and dehydrogenation products of toosendanin. (J to M) Tumor-derived cells were analyzed by flow cytometry for (J) CD3, (K) CD4/CD8, (L) Foxp3/CD25, and Dec 1, 2018 · X-ray analysis of toosendanin Crystals suitable for X-ray diffraction were grown from a mixture solution of methanol and water and mounted on a glass fibre for diffraction experiment. 05) to analyze ecdysteroid production Africa Trypanosomiasis remains a serious health problem, but the approved drugs for this disease are so few that novel trypanocidal compounds are demanded. With the skel-eton of tetracyclic triterpenoid, toosendanin gave relative low response and showed unsatisfactory sensitivity on UV detector. TSN and Ohchinin (OCN) were purchased from ChemFaces (Wuhan, China) (purity >98%) and used as experimental compounds and standards for ultra-performance liquid chromatography/mass spectrometry (UPLC/MS) (Akihisa et al. In this report, high-performance liquid chromatography (HPLC) separation using columns of … Sep 12, 2019 · Ultra-Performance Liquid Chromatography/Mass Spectrometry Analysis. [PMC free article] [Google Scholar] May 24, 2024 · The invasiveness of the cells is detected by a Transwell assay, western blotting, and RT-qPCR. Molecules 2013, 18, 1214412153; DOI:10. LT, MT, HT, DC, IC, BC are short for TSN low-dose group (5 mg/kg), TSN medium-dose group (10 mg/kg), TSN high-dose group (15 mg/kg), positive control group, infection control group, blank control group. TSN selectively inactivates phospho-STAT3 (Tyr-705); subsequent molecular docking and in vitro SPR analysis uncover TSN directly binds to the SH2 domain of STAT3. However, the hepatotoxicity caused by TSN or Melia-family plants containing TSN used in traditional Chinese medicine has been reported, and the mechanisms of TSN-induced liver injury … Apr 4, 2020 · The results indicate that the established analytical methods are suitable for detecting toosendanin and trans-anethole, and the methodology meets the requirements of biological sample testing methods. Previous studies reported that toosendanin possesses hepatotoxicity, but the mechanism remains unknown. In Vitro 27, 232–238 (2013). 1038/srep34225. HepG2 cCells were seeded in 24-well cell culture plates at a density of 2 × 10 4 cells/mL and incubated for Jun 15, 2024 · Toosendanin did not affect the expression of CD25 or secretion of IL-2 by activated T-cells, and not affect the expression of IL-4 and INF-γ. The anti-coccidial effect was investigated by treating different doses of TSN. The method could meet the requirement for general analysis and quality control of large bathes Characterization of a 2,3-oxidosqualene cyclase in the toosendanin biosynthetic pathway of Melia toosendan XufanLiana,†,XiuliZhangb,c,†, Fei Wangd, Xiaoning Wange Sep 1, 2023 · Toosendanin (TSN, C 30 H 38 O 11), a triterpenoid abundant in M. Toosendanin did not affect the phosphorylation of STAT5, ERK, AKT, P70S6K. The in vitro metabolism of toosendanin was studied by UHPLC-MS analysis. 4 x 10(-9) M. 02) nM. Finally, the results were confirmed using a xenograft tumor model that comprised of nude mice. Results from morphological analysis, annexin V staining, detection of caspases activity, and expressions of Bcl-2, Bax, and Fas indicated that the anticancer effects of toosendanin were associated with its induction of apoptosis via the mitochondria-dependent pathway in p53- and p53+ hepatocellular carcinoma cells. Although previously employed as a pesticide, recent studies have revealed its potential therapeutic value in treating various types of cancer. Jan 30, 2024 · Toosendanin (TSN) is a triterpenoid annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s, and finally ended by a melting curve analysis. Keywords: Melia azedarach L. Methods: The ecacy of toosendanin (TSN) in blocking autophagy ux was measured by western blot analysis of autophagy markers, and the uorescent imaging of RFP ‑GFP‑LC3 probe. Aug 8, 2022 · Effect of CTPS gene interference on the apoptosis of MKN- 45 cells induced by toosendanin (scale bar=25 μm). Statistical analysis by two-tailed Mann-Whitney t test (n = 6 mice). S1). azedarach L. B: Quantitative analysis of the rate of γH2AX positive cells (* P < 0. Jun 11, 2020 · In addition to the IR spectrum and the molecular formula C 30 H 38 O 11 determined by HR-FAB-MS ([M + H] + m/z 575. 0 (GraphPad Software, CA, USA). However, the hepatotoxicity caused by TSN or Melia-family plants containing TSN used in traditional Chinese medicine has been reported, and the mechanisms of TSN-induced liver injury (TILI) still remain largely unknown. Jun 15, 2024 · Anti-human CD3/CD28 antibodies stimulate T-cell proliferation through dual signalling (Martkamchan et al. , has been developed into an ascaris repellent in China. , 6 ( 2016 ) , p. For comparisons between two groups, we used Student's t tests, and for comparisons among three groups, we used one-way ANOVA. tative analysis of triterpenoids such as ginsenosides in botanical extracts. Oct 13, 2023 · Utilizing a bioassay-guided fractionation approach coupled with UHPLC-MS/MS analysis, we isolated and identified the triterpenoid compound toosendanin (TSN) as the active constituent responsible for MTE’s anti-osteoclastogenic activity. In search for trypanocidal principles from medicinal plants, we found MeOH extracts of Meliae Cortex with potent activity through the screening … Aug 7, 2017 · Integrated analysis of microRNA and mRNA expression profiles highlights the complex and dynamic behavior of toosendanin-induced liver injury in mice. Samples were well separated on an Agilent ZOBAX SB C18 column (4. Nov 18, 2016 · Representative images and statistical analysis of the luminescence are illustrated (* p < 0. We performed a functional screen to identify small-molecule compounds that can reverse macrophage-mediated immunosuppression in an in vitro tumor microenvironment system. , 1980). Network pharmacology identified mTOR as a key target of TSN, and surface plasmon resonance analysis confirmed TSN's direct binding to mTOR, underscoring mTOR's role in TSN's protective effects against ALI. Nov 9, 2023 · Statistical analysis. Analysis for (L) CD25, (M) IFN-γ, and (N) Ki67 expression in T cells. Researches on this compound could date back to 1953. Trichilin H (50) and toosendanin (10) with C-14/C-15-epoxide structures showed high cytotoxicity to human KB tumor cell lines in vitro. Furthermore, GO annotation analysis revealed 371 DEGs associated with metabolic processes (Fig. May 1, 2021 · Methods The efficacy of toosendanin (TSN) in blocking autophagy flux was measured by western blot analysis of autophagy markers, and the fluorescent imaging of RFP-GFP-LC3 probe. Left: Representative cell sorting. The samples were analyzed in triplicate, and toosendanin content in MAE was found to be 2. The apoptotic effects of toosendanin are partially mediated by activation of deoxycytidine kinase in HL-60 cells. Nude mice are injected with H226 (1×10 6) and received an intraperitoneal injection of toosendanin every 2 days for 21 days. May 13, 2020 · Introduction. Benshui Shu: Conceptualization, Formal analysis, Funding Integrated analysis of microRNA and mRNA expression profiles highlights the complex and dynamic behavior of toosendanin-induced liver injury in mice Article Full-text available for the quantitative analysis of toosendanin. Dec 3, 2022 · Toosendanin (TSN) is a Analysis of Cell Morphology. 5 Mar 15, 2023 · Toosendanin (TSN) is the main The results of qPCR analysis and western blotting assay showed that TSN-induced activation of protein kinase R-like endoplasmic Apr 24, 2023 · Toosendanin targeting eEF2 impedes Topoisomerase I & II protein translation to suppress esophageal squamous cell carcinoma growth. com×journal×molecules Article Metabolite Analysis of Toosendanin by an Ultrahigh Performance Jul 1, 2024 · The comparison of HE analysis, PAS analysis and AB-PAS analysis of cecum tissues in different groups. Toosendanin (TSN) is a triterpenoid derivative found in Melia toosendan Data Analysis All analyses but one were conducted with the GraphPad Prism 5. toosendan []. Its bioavailability in rats is low, which indicates the hepatotoxicity might be induced by its metabolites. Consequently, TSN blocks Oct 5, 2016 · Triterpenoid Toosendanin (TSN) exhibits a plenty of pharmacological effects in human and great values in agriculture. , 2017). Flow cytometric analysis revealed that treatment of U937 cells with Sep 15, 2020 · Toosendanin (TSN) (Fig. This quantitative approach showed good sensitivity, high accuracy, and stable repeatability. 2 per 100,000 population, glioma can be classified into high-grade (grade 3 and grade 4) and low-grade (grade 1 and grade 2) according to World Health Organization Feb 15, 2023 · Statistical analysis by Mann-Whitney t test (mean ± SEM). tenella infection was established. are significantly different; based on Tukey's test. Furthermore, Hoechst 33342 staining, flow cytometry, and western blotting analysis were performed to investigate the apoptotic activities of TSN. 4. The results of GO items, including biological process, cellular compound, and molecular function were visualized by bar plot, and the KEGG pathway enrichment results show in Jun 22, 2020 · Toosendanin (TSN) is a tetracyclic triterpenoid extracted from Melia toosendan Sieb, et Zucc, which primarily grows in specific areas of China. Jul 29, 2022 · Toosendanin activates caspase-1 and induces maturation of IL-1β to inhibit type 2 porcine reproductive and respiratory syndrome virus replication via an IFI16-dependent pathway Mingxin Zhang , 1 Chunni Lu , 2 Lizhan Su , 1 Feixiang Long , 1 Xia Yang , 1 Xiaofeng Guo , 1 Gaopeng Song , 3 Tongqing An , 4 Weisan Chen , 5 and Jianxin Chen 1 Oct 23, 2024 · Melia. toosendan is a commonly used herbal medicine with anti-bacterial, antiviral, anthelmintic, insecticidal, analgesic, antitumor, and anti-allergic [1,2,3]. B The analysis of tumor volumes, tumor weights, Sep 16, 2021 · Background Toosendanin (TSN) is a triterpenoid compound mainly used as an ascaris repellant. , has been found to suppress proliferation and induce apoptosis in a variety of human cancer cells. Sci Rep. (H) Tumor-derived cells were analyzed by flow cytometry analysis for IL-10 expression in tumor macrophages. The co-localization of autophagosomes and lysosomes was analyzed by fluorescent imaging. , 1999). Sep 30, 2013 · Toosendanin is the major bioactive component of Melia toosendan Sieb. Methods Different assays were performed to investigate the effects of TSN on the different glioma cell lines including U87MG and LN18. 05); (D) Histological analysis of U87 cell proliferation and apoptosis by Ki67 staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay (* p < 0. Western blot analysis was used to analyze expression levels of apoptosis-related protein Bcl-2, pro-apoptotic Bax, cytochrome c, caspase-3, −8 and −9 and PARP to Toosendanin (TSN) is a triterpenoid derivative found in Melia toosendan Sieb. The stability results of toosendanin in rat plasma are summarized in Table IV. com×journal×molecules Article Metabolite Analysis of Toosendanin by an Ultrahigh Performance This study established a high-performance liquid chromatography (HPLC) coupled with charged aerosol detector (CAD) method to determine toosendanin, the major active constituent in the fruit of M. However, with the improvement of public health protection, the incidence of ascariasis has been reduced considerably, resulting in limited medical application of TSN. Toosendanin had an IC50 of 56 nM (32 ng/ml) against LT, likely by inhibiting endocytosis and endosomal acidification. Control diets were prepared with carrier alone. Data analysis was determined using the Student’s two-tailed t-test or one-way ANOVA. Analysis of digestive enzyme activity revealed that TSN inhibited the activities of acetyl CoA carboxylase, lipase, α-amylase, and trypsin. 05 vs Sh-ctrl). It was found that toosendanin could be quickly metabolized by human liver microsomes and six metabolites were identified for the first time. Jul 1, 2021 · Meanwhile, large-scale chemical proteomics analysis showed that at least 84 proteins were modified by TSN RMs in rat liver, and the bioinformatics analysis revealed that TSN might induce hepatotoxicity through multi-target protein-protein interaction especially involved in energy metabolism. azedarach, is a limonoid compound that has been employed as a digestive tract parasiticide and an agricultural insecticide in the ancient world (Xu et al. 3390×molecules181012144 OPEN ACCESS molecules ISSN 14203049 www. The ethanol extract of MF was prepared as described previously (Jin et al. Feb 28, 2007 · Further analysis on the MS spectra showed M1, M2, and M3 are oxidative products and M6 is a dehydrogenation product, while M4 and M5 are oxidative and dehydrogenation products of toosendanin. We found that TSN acted as the ligand of WWOX and could interact with WWOX through a hydrophobic pocket formed by residues such as GLY10, PRO13, TYR14, TRP16, ALA9, GLY10, ASP11, LEU12 and TRP16. Nov 10, 2021 · HUVEC cells pre-treated with 1 μM toosendanin (B) or a series concentration of toosendanin (C) for 1 h were chilled at 4°C for 15 min and incubated with SFTSV (MOI = 5) for 1 h. Feb 1, 2010 · To resolve this dilemma, we reported the effects of well characterized toosendanin, using a sensitive and specific spinal cord cell-based assay, which validated toosendanin's activity in both BoNT serotypes A and E. 9,10,13,15 From various reports, ESI-MSn is known to be useful for structural analysis of saponins in medicinal plant extracts with excellent sensitivity and using only small amounts of the samples. ; uniform design; regression analysis; toosendanin; collecting time _____ INTRODUCTION Toosendanin (a common name in China is “chuan lian su” or “ku lian su”) is a natural triterpenoid comp ound Dec 1, 2022 · Methods The efficacy of toosendanin (TSN) in blocking autophagy flux was measured by western blot analysis of autophagy markers, and the fluorescent imaging of RFP-GFP-LC3 probe. Conclusions. Sep 1, 2023 · The results of the linear discriminant analysis effect size (LEfSe) analysis suggest that six bacterial genera contributed to the different structures of the larval bacterial community. 1) is a natural tetracyclic nortriterpenoid isolated from the medicinal herb Melia toosendan Sieb et Zucc, 6, 7 toosendan fructus has also been recorded as a little toxic drug in Chinese Pharmacopoeia. C: Cells treated with toosendanin for 48 h after transfection with CTPS interference vector. 5 D). Dec 1, 2021 · Integrated analysis of microRNA and mRNA expression profiles highlights the complex and dynamic behavior of toosendanin-induced liver injury in mice Sci. Jan 1, 2025 · Functional and pathway enrichment analysis revealed that Gene Ontology biological processes included ER stress response, apoptosis, and oxidative stress (Fig. The principal active components of the Melia. The IC 50 value was calculated using nonlinear regression for dose–response inhibition. Anti-cancer effects of crude extract from Melia toosendan Sieb. These results suggested that TSN was a selective prejunctional blocker. Feb 15, 2023 · Statistical analysis by one-way ANOVA. toosendan are flavonoids, triterpenoids, phenolic and alkaloids, among which toosendanin contains the highest content and is the main active component of the Melia. The co May 21, 2024 · The invasiveness of the cells is detected by a Transwell assay, western blotting, and RT-qPCR. 05). (I) Tumor lysates were subjected to enzyme-linked immunosorbent assay analyses for IL-10 and TGF-β expression. Data analysis was performed by Prism 7. A preliminary mech … Toosendanin (TSN), a triterpenoid isolated from Melia toosendan Sieb. KEGG analysis further indicated the significant presence of DEGs in various pathways, including fat digestion and absorption, protein digestion and absorption, and pancreatic secretion (Fig. 1), a triterpenoid compound, isolated from the bark of Melia toosendan (Meliaceae Juss: Melia Linn), has been used as a vermifuge against ascaris for decades, an insecticidal agent more than 2000 years in China as well (Shi and Li 2007; Tada et al. , which is traditionally used for treatment of abdominal pain and as an insecticide. 9 It has been reported that TSN exhibited well biological actions The efficacy of toosendanin (TSN) in blocking autophagy flux was measured by western blot analysis of autophagy markers, and the fluorescent imaging of RFP-GFP-LC3 probe. Toosendanin is a phytochemical found in the medicinal plant Melia toosendan Sieb. In addition, LC/MS and LC/MS/MS have proven to be the appropriate Toosendanin, bearing a furan ring, is a limonoid belonging to the group of tetranortriterpenoids. Rep. Right: Quantitative results (n = 5 mice per group). 2494), detailed analysis of NMR spectra (1D and 2D) and the comparison of spectroscopic data with reported ones [9, 10] revealed A-2-1 and -2 to be toosendanin (1), a hemiacetal triterpenoid derivative (Fig. 0 software package ( GraphPad 2007 ) for the Student–Newman–Keuls test ( P < 0. , 2013). determined by regression analysis. 7 x 10(-7) M and U937 was the most sensitive cell line with a IC(50) of 5. Statistical Analysis. Sci Rep 2016; 6 : 34225. Quantitative results (n = 3 human samples, mean ± SEM). 5. p < 0. (A) U87 cells were treated with increasing concentrations (5–100 nM) of TSN for 48 h and assessed for growth inhibition by the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay; (B) The effect of TSN on C6 cell proliferation was assessed as described in (A); (C) U87 cells were treated with 10 nM Jan 1, 2025 · Toosendanin alleviates acute lung injury by reducing pulmonary vascular barrier dysfunction mediated by endoplasmic reticulum stress through mTOR Author links open overlay panel Xiaocheng Mao a # , Cheng Wang b # , Hong Tang a # , Xiaohua Liu a , Caihui Wei a , Fang Yin a , Tianmei Fu a , Yangyang Fang a , Kuai Yu a , Zhanglin Zhang a Oct 1, 2024 · Toosendanin (TSN), a tetracyclic triterpenoid derived from Melia toosendan and M. Toosendanin has also been found to suppress proliferation and induce apoptosis in a variety of human cancer cells, possibly mediated through JNK signaling pathway. The co‑localization of autophagosomes and lysosomes was analyzed by uorescent imaging. [PMC free article] [Google Scholar] Ju J. 5 × 10 5 cells were seeded in 60 mm dishes containing 0, 5, 10 or 20 nM TSN supplemented media for 48 h or 72 h. springer. 19. All data are expressed as the mean ± standard deviation (SD) of at least three independent experiments. et Zucc were investigated in in Mar 1, 2023 · Toosendanin (TSN, 1, Fig. Aug 10, 2021 · Molecular docking analysis and SPR analysis confirmed that TSN had a strong binding ability with WWOX. Transcriptome analysis of toosendanin (TSN)-treated macrophages Organism: Homo sapiens (Taxonomy ID 9606) BioProject Accession: PRJNA804920 ID: 804920 Jul 20, 2016 · In this work, a simple and rapid high-performance liquid chromatography coupled with charged aerosol detector (HPLC-CAD) method was first developed for the quantitation of toosendanin, the major constituent of the dried fruit of Melia toosendan Sieb. Et Zucc. 1 Small-molecule toosendanin reverses macrophage-mediated immunosuppression to overcome glioblastoma resistance to immunotherapy F Yang, D Zhang, H Jiang, J Ye, L Zhang, SJ Bagley, J Winkler, Y Gong, Abstract. azedarach, demonstrates broad application prospects in cancer treatment. fraction and purified yeast V -ATPase, we found that toosendanin directly inhibited V -ATPase activity. Toosendanin is the major bioactive component of Melia toosendan Sieb. Sep 11, 2019 · Figure 1 UPLC-MS chromatograms of toosendanin, Ohchinin, and Melia Fructus (MF) ethanol extract. 05, min overlap = 3 and min enrichment ratio = 1. 4 C). Et Zucc (Meliaceae) or chinaberry. May 9, 2019 · Toosendanin modulates the expression levels of caspase and Bcl-2 family proteins . Nov 11, 2021 · HUVEC cells pre-treated with 1 μM toosendanin (B) or a series concentration of toosendanin (C) for 1 h were chilled at 4°C for 15 min and incubated with SFTSV (MOI = 5) for 1 h. Stability. However, toosendanin inhibited proliferation of anti-CD3/anti-CD28 mAbs-activated T-cells with IC50 of (10 ± 2. 1. In this study, we examined the effects of TSN … Thus, this study provides the direction for obtainment of toosendanin from chinaberry. mdpi. Jul 1, 2021 · The efficacy of toosendanin (TSN) in blocking autophagy flux was measured by western blot analysis of autophagy markers, and the fluorescent imaging of RFP-GFP-LC3 probe. However, the mechanism how TSN induces apoptosis remains poorly understood. Sep 30, 2013 · Further analysis on the MS spectra showed M1, M2, and M3 are oxidative products and M6 is a dehydrogenation product, while M4 and M5 are oxidative and dehydrogenation products of toosendanin. By applying cellular thermal shift assay (CETSA), immunoprecipitation-coupled LC-MS/MS analysis, and biotin-toosendanin pull-down assay, we confirmed the direct binding between toosendanin and V-ATPase. Toosendanin has been named in 1955, while its structure was identified 20 years later by mass spectrometry. (B) WB analysis of LC3B-II and SQSTM1/p62 protein levels in HeLa and Metabolite Analysis of Toosendanin by an Ultra-High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry Technique Jian-Lin Wu, Elaine Lai-Han Leung, Hua Zhou, Liang Liu * and Na Li * State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Using rat liver lysosome fraction and purified yeast V-ATPase, we found that toosendanin directly inhibited V-ATPase activity. , 2019). The results indicated that TSN treatment led to pathological changes in the midgut structure. 86 ± 0. [Google Scholar] May 6, 2022 · Methods: The efficacy of toosendanin (TSN) in blocking autophagy flux was measured by western blot analysis of autophagy markers, and the fluorescent imaging of RFP-GFP-LC3 probe. Toosendanin (TSN) inhibited glioblastoma (GBM) cell proliferation. com Oct 1, 2013 · Further analysis on the MS spectra showed M1, M2, and M3 are oxidative products and M6 is a dehydrogenation product, while M4 and M5 are oxidative and dehydrogenation products of toosendanin. (L to N) After incubation of sorted macrophages with CD3/CD28-activated human T cells for 3 days, cells were analyzed by flow cytometry. doi: 10. (A) The chemical structures of toosendanin (TSN) and Ohchinin (OCN) and the UPLC-MS chromatograms of TNS (upper panel) and OCN (lower panel) standards in single ion monitoring (SIM) mode. et Zucc. In this connection See full list on link. Toxicol. Toosendanin is a triterpenoid derivative containing a β-substituted furan ring . Mar 28, 2022 · Using rat liver lysosome fraction and purified yeast V-ATPase, we found that toosendanin directly inhibited V-ATPase activity. Toosendanin induces apoptosis through suppression of JNK signaling pathway in HL-60 cells. Jul 20, 2016 · The IC(50) values were less than 1. Feb 28, 2007 · High-performance liquid chromatography separation using columns of smaller particle size with tandem mass spectrometry (MS(n) was used for the rapid determination of toosendanin in botanical extracts and showed that MS(3) generated more characteristic ions that are useful for the identification of TSN in unknown samples. Intensity data were collected on an Oxford Diffraction Xcaliburâ„¢S Ultra (Oxford, UK) with CCD Area Detector at 100K with Enhance Ultra Cu-Kα radiation Statistical analysis by two-tailed Student’s t test (mean ± SEM, n = 5 or 6 mice per group). It lowered ROS levels, lipid peroxidation, endoplasmic reticulum (ER) stress, and apoptosis, both in vitro and in vivo. Sep 30, 2013 · Six metabolites obtained by incubating toosendanin with human live microsomes were examined and six of these metabolites were identified for the first time by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF/MS). toosendan. 6 mm × 250 mm, 5 μm) by isocratic elution using 33 % acetonitrile and Feb 10, 2024 · The quantitative analysis of toosendanin in the MAE was measured by developed HPLC/ELSD method. a The chemical structure of TSN. Toosendanin has certain toxicity and was mainly used in agriculture in the early stage. Mar 1, 2023 · Besides, the GO and KEGG enrichment analysis were exerted by the Metascape database, set the threshold as: p-value < 0. Moreover, their possible structures were deduced from the MS/MS spectral features. Although toosendanin (TSN) exerts antitumoral effects on various human cancer cells, its influence on gastric cancer (GC) is remains to be elucidated. Simultaneously, it upregulated superoxide dismutase and reduced malondialdehyde content. The Apr 15, 2019 · Toosendanin (TSN), a compound from Melia toosendan, exhibits severe hepatotoxicity, which restricts its clinical application. 4 B). 2488, calcd 575. tenella) in chicks. Recent studies have shown that it possesses antitumor effects in many types of tumor cells. Toosendanin modulates the expression levels of caspase and Bcl-2 family proteins. Sep 16, 2021 · The assays included colony formation, wound healing, and transwell assays. Toosendanin and its derivatives demonstrate high insecticidal activity and are importa … from publication: Metabolite Analysis of Toosendanin by an Ultra-High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry Technique | Toosendanin is the major bioactive To investigate the therapeutic effect of toosendanin (TSN) against Eimeria tenella (E. Sep 21, 2021 · Toosendanin (TSN) inhibited the proliferation of glioma cells. Nov 11, 2017 · In this study, we identify Toosendanin (TSN) is an effective inhibitor of STAT3, leading to the impediment of various oncogenic processes in osteosarcoma. 05; treatments compared by dose to the control) or the one-way analysis of variance (ANOVA) followed by Tukey multiple comparison procedure ( α = 0. Toosendanin; CAS Number: 58812-37-6; Linear Formula: C30H38O11; find AChemBlock-ADVH97AC91D3 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich May 1, 2007 · On further analysis, it was observed that TSN did not affect the propagation of impulses in nerve fibers, the resting potential of the muscle fibers or the ACh sensitivity of the muscle membrane (Shi et al. PLoS One 7, e52536 (2012). Toosendanin is a tetracyclic triterpene compound derived from toosendan fruit [21]. Statistical analysis by Mann-Whitney t test (mean ± For MS/MS analysis, targeted MS/MS mode was selected, and the collision cell energy was set at 10, 20, 30 and/or 40 eV. 04 mg/g. Statistical analysis was performed using Prism9 software (GraphPad, San Diego, CA, USA). Meanwhile, large-scale chemical proteomics analysis showed that at least 84 proteins were modified by TSN RMs in rat liver, and the bioinformatics analysis revealed that TSN might induce hepatotoxicity through multi-target protein-protein interaction especially involved in energy metabolism. Glioma is the most common and aggressive primary tumor in adult central nervous system (CNS) worldwide, accounting for approximately 30% of CNS tumors and 80% of brain malignancies. Metabolite Analysis of Toosendanin by an Ultra-High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry Technique Jian-Lin Wu, Elaine Lai-Han Leung, Hua Zhou, Liang Liu * and Na Li * State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, May 6, 2022 · The efficacy of toosendanin (TSN) in blocking autophagy flux was measured by western blot analysis of autophagy markers, and the fluorescent imaging of RFP-GFP-LC3 probe. et Zucc, and the content of toosendanin in the crude extract was measured by high performance liquid chromatography (HPLC). Kyoto Encyclopedia of Genes and Genomes pathway analysis showed TSN-related targets were involved in apoptosis, mTOR signaling, and Toll-like receptor pathways (Fig. Mar 28, 2022 · The identification of the autophagy inhibitor TSN. 05 was considered statistically significant. Then, lysosome function was determined by measuring the lysoso ‑ Apr 24, 2023 · Cell cycle and apoptosis analysis. of the Meliaceae family. (A) Results of high-content screening for chemical autophagy modulators. Although the LC–MS method showed obvi-ous advantage for its distinctive sensitivity and selectivity, it can hardly meet the requirement of the routine analysis Dec 21, 2021 · The recoveries of toosendanin and the IS from rat plasma were consistent, precise and reproducible. toosendan Sieb. TSN present in the medicinal plants was first isolated and established by spectroscopic methods. Integrated analysis of microRNA and mRNA expression profiles highlights the complex and dynamic behavior of toosendanin-induced liver injury in mice.